Author: Betty Sri Laksmi Jenie; Ridawati; Winiati Pudji Rahayu
Abstract: M.purpureus in tapioca liquid waste medium consisted of 2 percent tapioca and 0.15 percent ammonim nitrate produced the highest red pigment intensity (500 nm) either for extracellular pigment. Addition of tapioca solid waste into the medium increased the intensity of the intracellular pigment (11.79) compare to the addition of rice flour (8.53). Utilization of 2 percent tofu solid waste as nitrogen source resulted in lower intracellular pigment intensity (8.24) than ammonium nitrate (12.08). The optimum pigment production was achieved after 12 days fermentation. The red pigment was first detected in the medium on the third day of fermentation when the dried cell mass reached maximum. The intensity of the pigment continue to rise during prolonged fermentation while the dried cell mass began to decrease. The starch content decreased during fermentation and the sugar content was first increase for 3 days and then decrease. The solubility of the red pigment in water was affected by the carbon and nitrogen sources used in the medium. Tapioca solid waste and ammonium nitrate as well as temperature at 80 oC increased the pigment solubility.
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