Types and Functions of The Microscope

Compound Light Microscopy: functions to see small objects with bright field
Darkfield Microscope: observed microbial can not be seen with ordinary light microscope, no can be characterized by standard methods, or experience distortion after staining. This microscope condenser using dark field condenser is not allow the light transmitted through the specimen continue into the objective lens.
Phase-Contrast Microscopy: allowing examination in detail the internal structure of microbial life. Using a special condenser.
Differential Interference Contrast Microscopy: This microscope allows viewing of objects into 3 dimension. The light is broken down and recombined with special prism.
Fluorescence microscopy: using fluorescent materials thus giving the color, use a uv light or near uv. If the object is not naturally fluorescent, objects can be colored with a fluorescent material called a fluorochrome. Examples of auramine O to give yellow color with UV, so absorbed with Mycobacterium tuberculosis; fluorescein isothiocyanate (FITC) gives bright yellow color, is absorbed by Bacillus anthracis. Specialized techniques in microscopy is a technique called antibody-fluorescent or immunofluoresen, using antigen-antibody reaction.
Electron Microscopy: there are two types of transmission electron microscopy (TEM) and scanning electron microcopy (SEM). The difference between the two lies in the use. TEM is used to view the preparations ultra-thin slices, preparations can be colored. In the SEM specimens do not need to be sliced ​​thin with results 3-dimensional observations. Both the microscope can enlarge the shadow of the object of observation until tens of thousands of times, something that can not be done with ordinary light microscope. Lens on the microscope This form of the electric field.