Oxidative Stress In Liver Tissue Of Rat Induced By Chronicsystemic Hypoxia

Abstract: Adaptation mechanism to hypoxia in living organisms increases reactive oxygen species (ROS) formation that couldexceed the capacity of anti oxidant. Gluthatione (GSH) in which highest concentration present in liver, plays animportant role in maintaining the intracellular redox equilibrium and protect tissues from oxidative stress. The aim ofthis study was to observe tissue response of rat that was exposed to chronic systemic hypoxia by analyzing the oxidativestress in liver tissue. Twenty male Sprague-Dawley rats were induced by chronic systemic hypoxia by kept them inhypoxic chamber (10% O2:90% N2) for 1, 3, 7 and 14 day(s). All rats were sacrificed with ether anesthesia afterhypoxia treatment. Liver tissues were analyzed using parameters of oxidative stress, malondialdehyde (MDA) withtBARS test, and endogenous antioxidant, glutathione reduced form (GSH). The study showed that chronic systemichypoxia induction caused oxidative stress in liver tissue, which was shown by increased concentration of MDA in livertissue (nmol/mg liver tissue). Concentration of MDA in liver tissue was increased significantly on day-1, day-3, day-7and day-14 compared to control group (ANOVA, LSD, p<0.05). The differences between day-3, day-7 and day-14 wasnot significant. In contrast, liver GSH content (μg/mg liver protein) was progressively decreased significantly sinceday-1 of hypoxia until the end of experiment (ANOVA, LSD, p<0.05). Statistical analysis revealed that there is a strongcorrelation between MDA and GSH concentration in liver tissue (Pearson = - 0.993). It was concluded that oxidativestress present in liver tissue of rat induced by chronic systemic hypoxia.